Publications

Ji, M.; Arbel, M.; Zhang, L.; Freudiger, C.W.; Hou, S.S.; Lin, D.; Yang, X.; Bacskai, B.J.; Xie, X. Label-free imaging of amyloid plaques in Alzheimer’s disease with stimulated Raman scattering microscopy. Sci Adv 2018, 4(11), eaat7715 DOI: 10.1126/sciadv.aat7715
Fu, D.; Yang, W.; Xie, X.S. Label-free Imaging of Neurotransmitter Acetylcholine at Neuromuscular Junctions with Stimulated Raman Scattering. J Am Chem Soc 2017, 139(2), 583−586 DOI: 10.1021/jacs.6b10727
Yang, W.; Li, A.; Suo, Y.; Lu, F.; Xie, X.S. Simultaneous two-color stimulated Raman scattering microscopy by adding a fiber amplifier to a 2 ps OPO-based SRS microscope. Opt Lett 2017, 42(3), 523-526 DOI: 10.1364/OL.42.000523
Lu, F.; Calligaris, D.; Olubiyi, O.I.; Norton, I.; Yang, W.L.; Santagata, S.; Xie, X.; Golby, A.J.; Agar, N.Y.R. Label-Free Neurosurgical Pathology with Stimulated Raman Imaging. Cancer Res 2016, 76, 3451-3462 DOI: 10.1158/0008-5472.CAN-16-027
Tian, F.; Yang, W.; Mordes, D.A.; Wang, J.; Salameh, J.S.; Mok, J.; Chew, J.; Sharma, A.; Leno-Duran, E.; Suzuki-Uematsu, S.; Suzuki, N.; Han, S.S.; Lu, F.; Ji, M.; Zhang, R.; Liu, Y.; Strominger, J.; Shneider, N.A.; Petrucelli, L.; Xie, X.S.; Eggan, K. Monitoring peripheral nerve degeneration in ALS by label-free stimulated Raman scattering imaging. Nat Commun 2016, 7, 13283 DOI: 10.1038/ncomms13283
Lu, F.; Basu, S.; Igras, V.; Hoang, M.P.; Ji, M.; Fu, D.; Holtom, G.R.; Neel, V.A.; Freudiger, C.W.; Fisher, D.E.; Xie, X.S. Label-free DNA imaging in vivo with stimulated Raman scattering microscopy. Proc Natl Acad Sci USA 2015, 112(37), 11624-11629 DOI: 10.1073/pnas.1515121112
Ji, M.; Lewis, S.; Camelo-Piragua, S.; Ramkissoon, S.H.; Snuderl, M.; Venneti, S.; Fisher-Hubbard, A.; Garrard, M.; Fu, D.; Wang, A.C.; Heth, J.A.; Maher, C.O.; Sanai, N.; Johnson, T.D.; Freudiger, C.W.; Sagher, O.; Xie, X.S.; Orringer, D.A. Detection of human brain tumor infiltration with quantitative stimulated Raman scattering microscopy. Sci Transl Med 2015, 7(309), 309ra163-309ra163 DOI: 10.1126/scitranslmed.aab0195
Cheng, J.; Xie, X.S. Vibrational spectroscopic imaging of living systems: An emerging platform for biology and medicine. Science 2015, 350(6264), aaa8870-aaa8870 DOI: 10.1126/science.aaa8870
Fu, D.; Xie, X.S. Reliable Cell Segmentation Based on Spectral Phasor Analysis of Hyperspectral Stimulated Raman Scattering Imaging Data. Anal. Chem. 2014, 86(9), 4115-4119 DOI: 10.1021/ac500014b
Fu, D.; Yu, Y.; Folick, A.; Currie, E.; Farese, R.V.; Tsai, T.; Xie, X.S.; Wang, M.C. In Vivo Metabolic Fingerprinting of Neutral Lipids with Hyperspectral Stimulated Raman Scattering Microscopy . J. Am. Chem. Soc. 2014, 136(24), 8820-8828 DOI: 10.1021/ja504199s
Fu, D.; Zhou, J.; Zhu, W.S.; Manley, P.W.; Wang, Y.K.; Hood, T.; Wylie, A.; Xie, X.S. Imaging the intracellular distribution of tyrosine kinase inhibitors in living cells with quantitative hyperspectral stimulated Raman scattering. Nature Chem 2014, 6(7), 614-622 DOI: 10.1038/nchem.1961
Freudiger, C.W.; Yang, W.; Holtom, G.R.; Peyghambarian, N.; Xie, X.S.; Kieu, K.Q. Stimulated Raman scattering microscopy with a robust fibre laser source. Nat. Photonics 2014, 8(2), 153-159 DOI: 10.1038/nphoton.2013.360
Ji, M.; Orringer, D.A.; Freudiger, C.W.; Ramkissoon, S.; Liu, X.; Lau, D.; Golby, A.J.; Norton, I.; Hayashi, M.; Agar, N.Y.R.; Young, G.S.; Spino, C.; Santagata, S.; Camelo-Piragua, S.; Ligon, K.L.; Sagher, O.; Xie, X.S. Rapid, Label-Free Detection of Brain Tumors with Stimulated Raman Scattering Microscopy. Sci Transl Med 2013, 5(201), 201ra119-201ra119 DOI: 10.1126/scitranslmed.3005954
Fu, D.; Holtom, G.; Freudiger, C.; Zhang, X.; Xie, X.S. Hyperspectral Imaging with Stimulated Raman Scattering by Chirped Femtosecond Lasers. J. Phys. Chem. B 2013, 117(16), 4634-4640 DOI: 10.1021/jp308938t
Kong, L.; Ji, M.; Holtom, G.R.; Fu, D.; Freudiger, C.W.; Xie, X.S. Multicolor stimulated Raman scattering microscopy with a rapidly tunable optical parametric oscillator. Opt. Lett. 2013, 38(2), 145 DOI: 10.1364/ol.38.000145
Freudiger, C.W.; Pfannl, R.; Orringer, D.A.; Saar, B.G.; Ji, M.; Zeng, Q.; Ottoboni, L.; Ying, W.; Waeber, C.; Sims, J.R.; De Jager, P.L.; Sagher, O.; Philbert, M.A.; Xu, X.; Kesari, S.; Xie, X.S.; Young, G.S. Multicolored stain-free histopathology with coherent Raman imaging. Lab. Invest. 2012, 92(10), 1492-1502 DOI: 10.1038/labinvest.2012.109
Lu, F.; Ji, M.; Fu, D.; Ni, X.; Freudiger, C.W.; Holtom, G.; Xie, X.S. Multicolor stimulated Raman scattering microscopy. Mol Phys 2012, 110(15-16), 1927-1932 DOI: 10.1080/00268976.2012.695028
Lefrancois, S.; Fu, D.; Holtom, G.R.; Kong, L.; Wadsworth, W.J.; Schneider, P.; Herda, R.; Zach, A.; Sunney Xie, X.; Wise, F.W. Fiber four-wave mixing source for coherent anti-Stokes Raman scattering microscopy. Opt. Lett. 2012, 37(10), 1652 DOI: 10.1364/ol.37.001652
Zhang, X.; Roeffaers, M.B.J.; Basu, S.; Daniele, J.R.; Fu, D.; Freudiger, C.W.; Holtom, G.R.; Xie, X.S. Label-Free Live-Cell Imaging of Nucleic Acids Using Stimulated Raman Scattering Microscopy. Chemphyschem 2012, 13(4), 1054-1059 DOI: 10.1002/cphc.201100890
Fu, D.; Lu, F.; Zhang, X.; Freudiger, C.; Pernik, D.R.; Holtom, G.; Xie, X.S. Quantitative Chemical Imaging with Multiplex Stimulated Raman Scattering Microscopy. J. Am. Chem. Soc. 2012, 134(8), 3623-3626 DOI: 10.1021/ja210081h
Saar, B.G.; Johnston, R.S.; Freudiger, C.W.; Xie, X.S.; Seibel, E.J. Coherent Raman scanning fiber endoscopy. Opt. Lett. 2011, 36(13), 2396 DOI: 10.1364/ol.36.002396
Saar, B.G.; Contreras-Rojas, L.R.; Xie, X.S.; Guy, R.H. Imaging Drug Delivery to Skin with Stimulated Raman Scattering Microscopy. Mol. Pharm. 2011, 8(3), 969-975 DOI: 10.1021/mp200122w
Freudiger, C.W.; Roeffaers, M.B.J.; Zhang, X.; Saar, B.G.; Min, W.; Xie, X.S. Optical Heterodyne-Detected Raman-Induced Kerr Effect (OHD-RIKE) Microscopy. J. Phys. Chem. B 2011, 115(18), 5574-5581 DOI: 10.1021/jp1113834
Min, W.; Freudiger, C.W.; Lu, S.; Xie, X.S. Coherent Nonlinear Optical Imaging: Beyond Fluorescence Microscopy. Annu. Rev. Phys. Chem. 2011, 62(1), 507-530 DOI: 10.1146/annurev.physchem.012809.103512
Roeffaers, M.B.J.; Zhang, X.; Freudiger, C.W.; Saar, B.G.; Ruijven, M.v.; Dalen, G.v.; Xiao, C.; Xie, X.S. Label-free imaging of biomolecules in food products using stimulated Raman microscopy. J Biomed Opt 2011, 16(2), 021118 DOI: 10.1117/1.3516591
Freudiger, C.W.; Min, W.; Holtom, G.R.; Xu, B.; Dantus, M.; Sunney Xie, X. Highly specific label-free molecular imaging with spectrally tailored excitation-stimulated Raman scattering (STE-SRS) microscopy. Nature Photon 2011, 5(2), 103-109 DOI: 10.1038/nphoton.2010.294
Wang, M.C.; Min, W.; Freudiger, C.W.; Ruvkun, G.; Xie, X.S. RNAi screening for fat regulatory genes with SRS microscopy. Nat Meth 2011, 8(2), 135-138 DOI: 10.1038/nmeth.1556
Saar, B.G.; Freudiger, C.W.; Reichman, J.; Stanley, C.M.; Holtom, G.R.; Xie, X.S. Video-Rate Molecular Imaging in Vivo with Stimulated Raman Scattering. Science 2010, 330(6009), 1368-1370 DOI: 10.1126/science.1197236
Wang, K.; Freudiger, C.W.; Lee, J.H.; Saar, B.G.; Xie, X.S.; Xu, C. Synchronized time-lens source for coherent Raman scattering microscopy. Opt. Express 2010, 18(23), 24019 DOI: 10.1364/oe.18.024019
Xie, X.S. Single Molecule Spectroscopy in Chemistry, Physics and Biology. Springer Series in Chemical Physics 2010, DOI: 10.1007/978-3-642-02597-6
Saar, B.G.; Zeng, Y.; Freudiger, C.W.; Liu, Y.; Himmel, M.E.; Xie, X.S.; Ding, S. Label-Free, Real-Time Monitoring of Biomass Processing with Stimulated Raman Scattering Microscopy. Angew. Chem. Int. Ed. 2010, 49(32), 5476-5479 DOI: 10.1002/anie.201000900
Zeng, Y.; Saar, B.G.; Friedrich, M.G.; Chen, F.; Liu, Y.; Dixon, R.A.; Himmel, M.E.; Xie, X.S.; Ding, S. Imaging Lignin-Downregulated Alfalfa Using Coherent Anti-Stokes Raman Scattering Microscopy. Bioenerg. Res. 2010, 3(3), 272-277 DOI: 10.1007/s12155-010-9079-1
Freudiger, C.W.; Min, W.; Saar, B.G.; Xie, X.S. Stimulated Raman Scattering Microscopy for Label-Free Chemical Imaging. Optics & Photonics News 2009, 20(12), 30 DOI: 10.1364/opn.20.12.000030
Saar, B.G.; Holtom, G.R.; Freudiger, C.W.; Ackermann, C.; Hill, W.; Xie, X.S. Intracavity wavelength modulation of an optical parametric oscillator for coherent Raman microscopy. Opt. Express 2009, 17(15), 12532 DOI: 10.1364/oe.17.012532
Kieu, K.; Saar, B.G.; Holtom, G.R.; Xie, X.S.; Wise, F.W. High-power picosecond fiber source for coherent Raman microscopy. Opt. Lett. 2009, 34(13), 2051 DOI: 10.1364/ol.34.002051
Min, W.; Lu, S.; Rueckel, M.; Holtom, G.R.; Xie, X.S. Near-Degenerate Four-Wave-Mixing Microscopy. Nano Lett. 2009, 9(6), 2423-2426 DOI: 10.1021/nl901101g
Min, W.; Lu, S.; Holtom, G.R.; Xie, X.S. Triple-Resonance Coherent Anti-Stokes Raman Scattering Microspectroscopy. Chemphyschem 2009, 10(2), 344-347 DOI: 10.1002/cphc.200800502
Murugkar, S.; Evans, C.L.; Xie, X.S.; Anis, H. Chemically specific imaging of cryptosporidium oocysts using coherent anti-Stokes Raman scattering (CARS) microscopy. J. Microsc. 2009, 233(2), 244-250 DOI: 10.1111/j.1365-2818.2009.03114.x
Freudiger, C.W.; Min, W.; Saar, B.G.; Lu, S.; Holtom, G.R.; He, C.; Tsai, J.C.; Kang, J.X.; Xie, X.S. Label-Free Biomedical Imaging with High Sensitivity by Stimulated Raman Scattering Microscopy. Science 2008, 322(5909), 1857-1861 DOI: 10.1126/science.1165758
Evans, C.L.; Xie, X.S. Coherent Anti-Stokes Raman Scattering Microscopy: Chemical Imaging for Biology and Medicine. Annu Rev Anal Chem (Palo Alto Calif) 2008, 1(1), 883-909 DOI: 10.1146/annurev.anchem.1.031207.112754
Wright, A.J.; Poland, S.P.; Girkin, J.M.; Freudiger, C.W.; Evans, C.L.; Xie, X.S. Adaptive optics for enhanced signal in CARS microscopy. Opt. Express 2007, 15(26), 18209 DOI: 10.1364/oe.15.018209
Saar, B.G.; Park, H.; Xie, X.S.; Lavrentovich, O.D. Three-dimensional imaging of chemical bond orientation in liquid crystals by coherent anti- Stokes Raman scattering microscopy. Opt. Express 2007, 15(21), 13585 DOI: 10.1364/oe.15.013585
Evans, C.L.; Xu, X.; Kesari, S.; Xie, X.S.; Wong, S.T.; Young, G.S. Chemically-selective imaging of brain structures with CARS microscopy. Opt. Express 2007, 15(19), 12076 DOI: 10.1364/oe.15.012076
Nan, X.; Potma, E.O.; Xie, X.S. Nonperturbative Chemical Imaging of Organelle Transport in Living Cells with Coherent Anti-Stokes Raman Scattering Microscopy. Biophys. J. 2006, 91(2), 728-735 DOI: 10.1529/biophysj.105.074534
Ganikhanov, F.; Evans, C.L.; Saar, B.G.; Xie, X.S. High-sensitivity vibrational imaging with frequency modulation coherent anti-Stokes Raman scattering (FM CARS) microscopy. Opt. Lett. 2006, 31(12), 1872 DOI: 10.1364/ol.31.001872
Nan, X.; Tonary, A.M.; Stolow, A.; Xie, X.S.; Pezacki, J.P. Intracellular Imaging of HCV RNA and Cellular Lipids by Using Simultaneous Two-Photon Fluorescence and Coherent Anti-Stokes Raman Scattering Microscopies. Chembiochem 2006, 7, 1895-1897 DOI:
Légaré, F.; Evans, C.L.; Ganikhanov, F.; Xie, X.S. Towards CARS Endoscopy. Opt. Express 2006, 14(10), 4427 DOI: 10.1364/oe.14.004427
Ganikhanov, F.; Carrasco, S.; Sunney Xie, X.; Katz, M.; Seitz, W.; Kopf, D. Broadly tunable dual-wavelength light source for coherent anti-Stokes Raman scattering microscopy. Opt. Lett. 2006, 31(9), 1292 DOI: 10.1364/ol.31.001292
Rakic, B.; Sagan, S.M.; Noestheden, M.; Bélanger, S.; Nan, X.; Evans, C.L.; Xie, X.S.; Pezacki, J.P. Peroxisome Proliferator-Activated Receptor α Antagonism Inhibits Hepatitis C Virus Replication. Chem. Biol. 2006, 13(1), 23-30 DOI: 10.1016/j.chembiol.2005.10.006
Potma, E.O.; Evans, C.L.; Xie, X.S. Heterodyne coherent anti-Stokes Raman scattering (CARS) imaging. Opt. Lett. 2006, 31(2), 241 DOI: 10.1364/ol.31.000241
Evans, C.L.; Potma, E.O.; Puoris'haag, M.; Cote, D.; Lin, C.P.; Xie, X.S. Chemical imaging of tissue in vivo with video-rate coherent anti-Stokes Raman scattering microscopy. Proc. Natl. Acad. Sci. U.S.A. 2005, 102(46), 16807-16812 DOI: 10.1073/pnas.0508282102
Kou, S.C.; Cherayil, B.J.; Min, W.; English, B.P.; Xie, X.S. Single-Molecule Michaelis−Menten Equations. J. Phys. Chem. B 2005, 109(41), 19068-19081 DOI: 10.1021/jp051490q
Krug, J.T.; Sánchez, E.J.; Xie, X.S. Fluorescence quenching in tip-enhanced nonlinear optical microscopy. Appl. Phys. Lett. 2005, 86(23), 233102 DOI: 10.1063/1.1935769
Potma, E.O.; Xie, X.S. Direct Visualization of Lipid Phase Segregation in Single Lipid Bilayers with Coherent Anti-Stokes Raman Scattering Microscopy. Chemphyschem 2005, 6(1), 77-79 DOI: 10.1002/cphc.200400390
Evans, C.L.; Potma, E.O.; Xie, X.S. Coherent anti-Stokes Raman scattering spectral interferometry: determination of the real and imaginary components of nonlinear susceptibility χ^(3) for vibrational microscopy. Opt. Lett. 2004, 29(24), 2923 DOI: 10.1364/ol.29.002923
Potma, E.O.; Xie, X.S. CARS Microscopy for Biology and Medicine. Optics & Photonics News 2004, 15(11), 40 DOI: 10.1364/opn.15.11.000040
Potma, E.O.; Xie, X.S.; Muntean, L.; Preusser, J.; Jones, D.; Ye, J.; Leone, S.R.; Hinsberg, W.D.; Schade, W. Chemical Imaging of Photoresists with Coherent Anti-Stokes Raman Scattering (CARS) Microscopy. J. Phys. Chem. B 2004, 108(4), 1296-1301 DOI: 10.1021/jp030903p
Cheng, J.; Xie, X.S. Coherent Anti-Stokes Raman Scattering Microscopy: Instrumentation, Theory, and Applications. J. Phys. Chem. B 2004, 108(3), 827-840 DOI: 10.1021/jp035693v
Nan, X.; Yang, W.Y.; Xie, X.S. CARS Microscopy: Lights Up Lipids in Living Cells. Biophotonics Int. 2004, 11(44) DOI:
Rech, I.; Luo, G.; Ghioni, M.; Yang, H.; Xie, X.; Cova, S. Photon-Timing Detector Module for Single-Molecule Spectroscopy With 60-ps Resolution. IEEE J Sel Top Quantum Electron 2004, 10(4), 788-795 DOI: 10.1109/jstqe.2004.833975
Nan, X.; Cheng, J.; Xie, X.S.X. Vibrational imaging of lipid droplets in live fibroblast cells with coherent anti-Stokes Raman scattering microscopy. The Journal of Lipid Research 2003, 44(11), 2202-2208 DOI: 10.1194/jlr.d300022-jlr200
Potma, E.O.; Xie, X.S. Detection of single lipid bilayers with coherent anti-Stokes Raman scattering (CARS) microscopy. J Raman Spectrosc 2003, 34(9), 642-650 DOI: 10.1002/jrs.1045
Potma, E.O.; Evans, C.; Xie, X.S.; Jones, R.J.; Ye, J. Picosecond-pulse amplification with an external passive optical cavity. Opt. Lett. 2003, 28(19), 1835 DOI: 10.1364/ol.28.001835
Cheng, J.; Pautot, S.; Weitz, D.A.; Xie, X.S. Ordering of water molecules between phospholipid bilayers visualized by coherent anti-Stokes Raman scattering microscopy. Proc. Natl. Acad. Sci. U.S.A. 2003, 100(17), 9826-9830 DOI: 10.1073/pnas.1732202100
Hartschuh, A.; Sánchez, E.J.; Xie, X.S.; Novotny, L. High-Resolution Near-Field Raman Microscopy of Single-Walled Carbon Nanotubes. Phys. Rev. Lett. 2003, 90(9) DOI: 10.1103/physrevlett.90.095503
Cheng, J.; Volkmer, A.; Book, L.D.; Xie, X.S. Multiplex Coherent Anti-Stokes Raman Scattering Microspectroscopy and Study of Lipid Vesicles. J. Phys. Chem. B 2002, 106, 8493 DOI:
Cheng, J.; Potma, E.O.; Xie, X.S. Coherent Anti-Stokes Raman Scattering Correlation Spectroscopy: Probing Dynamical Processes with Chemical Selectivity. J. Phys. Chem. A 2002, 106, 8561 DOI:
Jones, D.J.; Potma, E.O.; Cheng, J.; Burfeindt, B.; Pang, Y.; Ye, J.; Xie, X.S. Synchronization of two passively mode-locked, picosecond lasers within 20 fs for coherent anti-Stokes Raman scattering microscopy. Rev. Sci. Instrum 2002, 73, 2843 DOI:
Cheng, J.; Xie, X.S. Green's function formulation for third-harmonic generation microscopy. JOSAB 2002, 19, 1604 DOI:
Potma, E.O.; Jones, D.J.; Cheng, J.; Xie, X.S.; Ye, J. High-sensitivity coherent anti-Stokes Raman scattering microscopy with two tightly synchronized picosecond lasers. Optics Lett 2002, 27, 1168 DOI:
Cheng, J.; Jia, Y.K.; Zheng, G.; Xie, X.S. Laser-Scanning Coherent Anti-Stokes Raman Scattering Microscopy and Applications to Cell Biology. Biophys. J 2002, 83, 502 DOI:
Cheng, J.; Volkmer, A.; Xie, X.S. Theoretical and experimental characterization of coherent anti-Stokes Raman scattering microscopy. JOSA B 2002, 19, 1363 DOI:
Volkmer, A.; Book, L.D.; Xie, X.S. Time-resolved coherent anti-Stokes Raman scattering microscopy: Imaging based on Raman free induction decay. Appl. Phys. Lett 2002, 80, 1505 DOI:
Cheng, J.; Book, L.D.; Xie, X.S. Polarization coherent anti-Stokes Raman scattering microscopy. Opt. Lett. 2001, 26(17), 1341 DOI: 10.1364/ol.26.001341
Volkmer, A.; Cheng, J.; Xie, X.S. Vibrational Imaging with High Sensitivity via Epidetected Coherent Anti-Stokes Raman Scattering Microscopy. Phys. Rev. Lett. 2001, 87(2) DOI: 10.1103/physrevlett.87.023901
Cheng, J.; Volkmer, A.; Book, L.D.; Xie, X.S. An Epi-Detected Coherent Anti-Stokes Raman Scattering (E-CARS) Microscope with High Spectral Resolution and High Sensitivity. J. Phys. Chem. B 2001, 105(7), 1277-1280 DOI: 10.1021/jp003774a
Zumbusch, A.; Holtom, G.R.; Xie, X.S. Vibrational Microscopy Using Coherent Anti-Stokes Raman Scattering. Phys. Rev. Lett 1999, 82, 4142 DOI: