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Xiaoliang Sunney Xie

Lee Shau-kee Professor, Peking University

Head, Faculty of Sciences, Peking University

Address: Peking University, No.2 Integrated Science Research Building, Rm. 105, Yiheyuan Road, No. 5, Haidian District, Beijing, P. R. China, 100871

Email: sunneyxie@pku.edu.cn

Introduction

Prof. Xiaoliang Sunney Xie is a world renown biophysical chemist. He received his BSc in chemistry from Peking University in 1984, and his PhD in physical chemistry in 1990 from UC San Diego. After a career at Pacific Northwest National Laboratory, he became the first tenured professor at Harvard University among Chinese Scholars who came to the USA since the Reform in China. As a pioneer of single molecule biophysical chemistry, coherent Raman scattering microscopy, and single cell genomics, he made major contributions to the emergence of these fields. Furthermore, Xie has made significant advances on medical applications of label-free optical imaging and single cell genomics. In particular, his inventions have been used in in vitro fertilization and have benefited hundreds of couples in China in avoiding the transmission of their monogenic diseases to their newborns. In addition, Prof. Xie has trained more than 100 graduate students and postdocs, many of whom have become professors at more than 40 major universities around the world. Since 2010, he has been the Director of Biodynamics Optical Imaging Center (BIOPIC) at Peking University, and in 2016 he became the Director of Beijing Advanced Innovation Center for Genomics (ICG). Prof. Xie is a member of the US National Academy of Sciences and the US National Academy of Medicine. Among his numerous honors are the Albany Medical Center Prize in Medicine and Biomedical Research, the American Chemical Society’s Peter Debye Award and the Biophysical Society Founders’ Award.
As of July 2023, Prof. Xie has published over 230 papers, and H-Index is 127. Prof. Xie’s current research interests include scientific research, technological research, and medical research.
  • Scientific: Single molecule enzymology, Single molecule biophysical chemistry, Gene expression and regulation, Epigenetics, Mechanism of cell differentiation and reprogramming, Genomic instability;
  • Technological: Single molecule imaging, Single cell genomics, Coherent Raman scattering microscopy, DNA sequencing;
  • Medical: Preimplantation genetic screening and diagnosis in in vitro fertilization, Early cancer diagnosis

Education

Ph.D. Chemistry University of California San Diego, with John D. Simon, 1990
B.S. Chemistry Peking University, Beijing, P. R. China, 1984

Professional Appointments

2020 - present Director, Changping Laboratory
2019 - present Head of Faculty of Sciences, Peking University
2018 - present Lee Shau-kee Professor, Peking University
2016 - 2022 Director, Beijing Advanced Innovation Center for Genomics, Peking University
2010 - 2022 Director, Biomedical Pioneering Innovation Center, Peking University
2009 - 2014 Cheung Kong Visiting Professor, Biodynamic Optical Imaging Center, Peking University
2009 - 2018 Mallinckrodt Professor of Chemistry and Chemical Biology, Harvard University
1999 - 2009 Professor of Chemistry, Harvard University, Department of Chemistry and Chemical Biology
1992 - 1998 Senior Research Scientist to Chief Scientist, Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory
1990 - 1992 Postdoctoral Fellow, University of Chicago, with Professor Graham R. Fleming

Honors

Selected Society fellowships
2023 Member of Chinese Academy of Science
2017 Foreign Member of Chinese Academy of Science
2016 Member of US National Academy of Medicine
2014 Fellow of Optical Society of America
2013 Humboldt Fellow, Germany
2012 Fellow of the American Academy of Microbiology
2011 Member of US National Academy of Sciences
2010 Honorary Fellow of the Chinese Chemical Society
2008 Fellow of the American Physical Society
2008 Fellow of American Academy of Arts & Sciences
2006 Fellow of American Association for the Advancement of Science
2006 Fellow of Biophysical Society
Selected Awards
2022 Zhong Guan Chun Outstanding Contribution Prize
2017 Qiu Shi Science and Technology Prize
2015 Albany Prize in Medicine and Biomedical Research, Albany Medical College
2015 Peter Debye Award in Physical Chemistry, American Chemical Society
2013 NIH Director’s Pioneer Award (Second time)
2013 Ellis R. Lippincott Award, Optical Society of America
2012 Harrison Howe Award, Rochester Section of American Chemical Society
2012 Founders Award, Biophysical Society
2009 E.O. Lawrence Award, U.S. Department of Energy
2008 Berthold Leibinger Future Prize, Leibinger Foundation, Germany
2007 Willis E. Lamb Award for Laser Science and Quantum Optics
2004 NIH Director’s Pioneer Award (First time)
2003 Raymond and Beverly Sackler Prize in the Physical Sciences, Israel
1996 Coblentz Award, Coblentz Society
Selected Named Lectureships
2020 Biophsycial Society National Lecture
2018 Baetjer Colloquium Lectureship, Princeton University
2017 George Pimentel Lecture, UC Berkeley
2016 Richard Bernstein Lecture, UCLA
2016 Linnett Lecture, Cambridge University
2015 Mendel Lecture, Masaryk Univ. in Brno, Czech Republic
2014 Chilton Lecture, UT Southwestern
2014 Marker Lecture, Pennsylvania State University
2014 Andrew Albrecht Lecture, Cornell
2014 Karl Friedrich Bonhoeffer Lecture, Max Planck Institute for Biophysical Chemistry
2012 Harkins Lecture, Univ. of Chicago
2011 Purves Lecture, McGill Univ., Montreal
2011 Guthikonda Memorial Lecture, Columbia Univ
2010 Dolan Pritchett Memorial Lecture, Univ. of Pennsylvania
2009 Einstein Professorship of the Chinese Academy of Sciences
2008 Lawrence Bragg Lecture, Cambridge Univ., Cavendish Laboratory
2008 Mueller Lecture, Purdue University
2008 Gordon Lecture, Univ. of Washington
2007 Chan Lecture, UC Berkeley
2007 Joseph Coleman Memorial Lecture, Yale
2007 Noyes Distinguished Lecture, Univ. of Texas at Austin
2007 Georges Smets Chair, Université catholique de Louvain, Belgium
2007 Dow Lecture, Univ. of British Columbia
2006 Marker Lecture, Pennsylvania State Univ.
2006 Wang Ying-lai Memorial Lecture, Univ. of Texas Medical Branch, Galveston
2005 Meyerhof Lecture, Max Planck Institute for Medical Research, Heidelberg, Germany
2005 PC Cross Lecture, Univ. of Washington, 2005; Noyes Lecture, Univ. of Rochester

Selected Professional Activities

Vice Chair, Academic Committee of Peking University, 2019-

Member, Scientific Advisory Board, Center of Brian Science at Harvard, 2008-2018

Member, Scientific Committee, Future Science Prize in China, 2016-2018

Member, Rowland Institute Advisory Board, Harvard University, 2002-2010

Member of Editorial Boards: Quart. Rev. Biophy. 2021-; J. Ass. Reprod. Genetics 2021-; Chem. Rev. 2015-2017; Science, 2009-2011; Ann. Rev. Phys. Chem. 2002-2007; Acc. Chem. Res. 2001-2006; J. Phys. Chem. 2000-2008; J. Microscopy 1999-2007; Single Molecules 1999-2002; Biophysical J. 2000-2001

Notable Meeting Organization

Founding co-chair Gordon Conference series on "Single Cell Genomics", 2022
Founding co-chair "Single Cell Omics" annual symposium, 2019-2022
Founding co-chair

Symposium on "New Advances in Optical Imaging of Live Cells and Organisms",

Cold Spring Harbor Asia, Suzhou, China, 2011 and 2013

Co-organizer Janelia Farm Conference, "Single Molecules Meet System Biology", HHMI, 2011
Co-organizer 355th Xiangshan Science Conference, "Single-Molecule Imaging, Spectroscopy and Manipulation of Biological Systems", Beijing, 2009
Founding co-chair Gordon Conference series on "Single Molecule Approaches to Biology", 2006
Organizer 1st-7th Annual Coherent Raman Microscopy Workshop, Harvard, Cambridge, 2004–2010

Full List of Journal Articles

236. Marcotte, H.; Cao, Y.; Zuo, F.; Simonelli, L.; Sammartino, J.C.; Pedotti, M.; Sun, R.; Cassaniti, I.; Hagbom, M.; Piralla, A.; Yang, J.; Du, L.; Percivalle, E.; Bertoglio, F.; Schubert, M.; Abolhassani, H.; Sherina, N.; Guerra, C.; Borte, S.; Rezaei, N.; Kumagai-Braesch, M.; Xue, Y.; Su, C.; Yan, Q.; He, P.; Grönwall, C.; Klareskog, L.; Calzolai, L.; Cavalli, A.; Wang, Q.; Robbiani, D.F.; Hust, M.; Shi, Z.; Feng, L.; Svensson, L.; Chen, L.; Bao, L.; Baldanti, F.; Xiao, J.; Qin, C.; Hammarström, L.; Yang, X.; Varani, L.; Xie, X.S.; Pan-Hammarström, Q. Conversion of monoclonal IgG to dimeric and secretory IgA restores neutralizing ability and prevents infection of Omicron lineages. Proc. Natl. Acad. Sci. U.S.A. 2014, 121(3), e2315354120 DOI: 10.1073/pnas.2315354120

235. Cao, Y.; Bai, Y.; Yuan, T.; Song, L.; Fan, Y.; Ren, L.; Song, W.; Peng, J.; An, R.; Gu, Q.; Zheng, Y.; Xie, X.S. Single-cell bisulfite-free 5mC and 5hmC sequencing with high sensitivity and scalability. Proc. Natl. Acad. Sci. U.S.A. 2023, 120(49), e2310367120 DOI: 10.1073/pnas.2310367120.

234. Xie, X.S. Round-Trip Journey of a Physical Chemist. J. Phys. Chem. B 2023, 127(37), 7800-7809 DOI: 10.1021/acs.jpcb.3c05597.

233. Hu, M.; Zhang, Y.; Yuan, Y.; Ma, W.; Zheng, Y.; Gu, Q.; Xie, X. S. Correlated protein modules revealing functional coordination of interacting proteins are detected by single-cell proteomics. J. Phys. Chem. B 2023, 127 (27), 6006-6014. DOI: 10.1021/acs.jpcb.3c00014.

232. Cao, Y.; Jian, F.; Wang, J.; Yu, Y.; Song, W.; Yisimayi, A.; Wang, J.; An, R.; Chen, X.; Zhang, N.; et al. Imprinted SARS-CoV-2 humoral immunity induces convergent Omicron RBD evolution. Nature 2023, 614 (7948), 521-529. DOI: 10.1038/s41586-022-05644-7.

231. Chapman, A. R.; Lee, D. F.; Cai, W.; Ma, W.; Li, X.; Sun, W.; Xie, X. S. Correlated gene modules uncovered by high-precision single-cell transcriptomics. Proc. Natl. Acad. Sci. U.S.A. 2022, 119 (51), e2206938119. DOI: 10.1073/pnas.2206938119.

230. Cao, Y.; Jian, F.; Zhang, Z.; Yisimayi, A.; Hao, X.; Bao, L.; Yuan, F.; Yu, Y.; Du, S.; Wang, J.; et al. Rational identification of potent and broad sarbecovirus-neutralizing antibody cocktails from SARS convalescents. Cell Rep. 2022, 41 (12). DOI: 10.1016/j.celrep.2022.111845.

229. Cao, Y.; Song, W.; Wang, L.; Liu, P.; Yue, C.; Jian, F.; Yu, Y.; Yisimayi, A.; Wang, P.; Wang, Y.; et al. Characterization of the enhanced infectivity and antibody evasion of Omicron BA.2.75. Cell Host Microbe 2022, 30 (11), 1527-1539.e1525. DOI: 10.1016/j.chom.2022.09.018.

228. Jian, F.; Yu, Y.; Song, W.; Yisimayi, A.; Yu, L.; Gao, Y.; Zhang, N.; Wang, Y.; Shao, F.; Hao, X.; et al. Further humoral immunity evasion of emerging SARS-CoV-2 BA.4 and BA.5 subvariants. Lancet Infect. Dis. 2022, 22 (11), 1535-1537. DOI: 10.1016/S1473-3099(22)00642-9.

227. Wu, H.; Li, X.; Jian, F.; Yisimayi, A.; Zheng, Y.; Tan, L.; Xing, D.; Xie, X. S. Highly sensitive single-cell chromatin accessibility assay and transcriptome coassay with METATAC. Proc. Natl. Acad. Sci. U.S.A. 2022, 119 (40), e2206450119. DOI: 10.1073/pnas.2206450119.

226. Cao, Y.; Yisimayi, A.; Jian, F.; Song, W.; Xiao, T.; Wang, L.; Du, S.; Wang, J.; Li, Q.; Chen, X.; et al. BA.2.12.1, BA.4 and BA.5 escape antibodies elicited by Omicron infection. Nature 2022, 608 (7923), 593-602. DOI: 10.1038/s41586-022-04980-y.

225. Cao, Y.; Wang, J.; Jian, F.; Xiao, T.; Song, W.; Yisimayi, A.; Huang, W.; Li, Q.; Wang, P.; An, R.; et al. Omicron escapes the majority of existing SARS-CoV-2 neutralizing antibodies. Nature 2021, 602 (7898), 657-663. DOI: 10.1038/s41586-021-04385-3.

224. Cao, Y.; Hao, X.; Wang, X.; Wu, Q.; Song, R.; Zhao, D.; Song, W.; Wang, Y.; Yisimayi, A.; Wang, W.; et al. Humoral immunogenicity and reactogenicity of CoronaVac or ZF2001 booster after two doses of inactivated vaccine. Cell Res. 2022, 32 (1), 107-109. DOI: 10.1038/s41422-021-00596-5.

223. Du, S.; Liu, P.; Zhang, Z.; Xiao, T.; Yasimayi, A.; Huang, W.; Wang, Y.; Cao, Y.; Xie, X. S.; Xiao, J. Structures of SARS-CoV-2 B.1.351 neutralizing antibodies provide insights into cocktail design against concerning variants. Cell Res. 2021, 31 (10), 1130-1133. DOI: 10.1038/s41422-021-00555-0.

222. Cao, Y.; Yisimayi, A.; Bai, Y.; Huang, W.; Li, X.; Zhang, Z.; Yuan, T.; An, R.; Wang, J.; Xiao, T.; et al. Humoral immune response to circulating SARS-CoV-2 variants elicited by inactivated and RBD-subunit vaccines. Cell Res. 2021, 31 (7), 732-741. DOI: 10.1038/s41422-021-00514-9.

221. Xing, D.; Tan, L.; Chang, C.-H.; Li, H.; Xie, X. S. Accurate SNV detection in single cells by transposon-based whole-genome amplification of complementary strands. Proc. Natl. Acad. Sci. U.S.A. 2021, 118 (8), e2013106118. DOI: 10.1073/pnas.2013106118.

220. Tan, L.; Ma, W.; Wu, H.; Zheng, Y.; Xing, D.; Chen, R.; Li, X.; Daley, N.; Deisseroth, K.; Xie, X. S. Changes in genome architecture and transcriptional dynamics progress independently of sensory experience during post-natal brain development. Cell 2021, 184 (3), 741-758.e717. DOI: 10.1016/j.cell.2020.12.032.

219. Chen, C.; Li, J.; Di, L.; Jing, Q.; Du, P.; Song, C.; Li, J.; Li, Q.; Cao, Y.; Xie, X. S.; et al. MINERVA: a facile strategy for SARS-CoV-2 whole-genome deep sequencing of clinical samples. Mol. Cell 2020, 80 (6), 1123-1134.e1124. DOI: 10.1016/j.molcel.2020.11.030.

218. Du, S.; Cao, Y.; Zhu, Q.; Yu, P.; Qi, F.; Wang, G.; Du, X.; Bao, L.; Deng, W.; Zhu, H.; et al. Structurally resolved SARS-CoV-2 antibody shows high efficacy in severely Infected hamsters and provides a potent cocktail pairing strategy. Cell 2020, 183 (4), 1013-1023.e1013. DOI: 10.1016/j.cell.2020.09.035.

217. Cao, Y.; Su, B.; Guo, X.; Sun, W.; Deng, Y.; Bao, L.; Zhu, Q.; Zhang, X.; Zheng, Y.; Geng, C.; et al. Potent neutralizing antibodies against SARS-CoV-2 identified by high-throughput single-cell sequencing of convalescent patients' B cells. Cell 2020, 182 (1), 73-84.e16. DOI: 10.1016/j.cell.2020.05.025.

216. Su, Q. P.; Zhao, Z. W.; Meng, L.; Ding, M.; Zhang, W.; Li, Y.; Liu, M.; Li, R.; Gao, Y. Q.; Xie, X. S.; et al. Superresolution imaging reveals spatiotemporal propagation of human replication foci mediated by CTCF-organized chromatin structures. Proc. Natl. Acad. Sci. U.S.A. 2020, 117 (26), 15036-15046. DOI: 10.1073/pnas.2001521117.

215. Di, L.; Fu, Y.; Sun, Y.; Li, J.; Liu, L.; Yao, J.; Wang, G.; Wu, Y.; Lao, K.; Lee, R. W.; et al. RNA sequencing by direct tagmentation of RNA/DNA hybrids. Proc. Natl. Acad. Sci. U.S.A. 2020, 117 (6), 2886-2893. DOI: 10.1073/pnas.1919800117.

214. Huang, L.; Bogale, B.; Tang, Y.; Lu, S.; Xie, X. S.; Racowsky, C. Noninvasive preimplantation genetic testing for aneuploidy in spent medium may be more reliable than trophectoderm biopsy. Proc. Natl. Acad. Sci. U.S.A. 2019, 116 (28), 14105-14112. DOI: 10.1073/pnas.1907472116.

213. Xiong, L.; Huang, L.; Tian, F.; Lu, S.; Xie, X. S. Bayesian model for accurate MARSALA (mutated allele revealed by sequencing with aneuploidy and linkage analyses). J. Assist. Reprod. Genet. 2019, 36 (6), 1263-1271. DOI: 10.1007/s10815-019-01451-8.

212. Tan, L.; Xing, D.; Daley, N.; Xie, X. S. Three-dimensional genome structures of single sensory neurons in mouse visual and olfactory systems. Nat. Struct. Mol. Biol. 2019, 26 (4), 297-307. DOI: 10.1038/s41594-019-0205-2.

211. Ji, M.; Arbel, M.; Zhang, L.; Freudiger, C. W.; Hou, S. S.; Lin, D.; Yang, X.; Bacskai, B. J.; Xie, X. S. Label-free imaging of amyloid plaques in Alzheimer's disease with stimulated Raman scattering microscopy. Sci. Adv. 2018, 4 (11), eaat7715. DOI: 10.1126/sciadv.aat7715.

210. Tan, L.; Xing, D.; Chang, C. H.; Li, H.; Xie, X. S. Three-dimensional genome structures of single diploid human cells. Science 2018, 361 (6405), 924-928. DOI: 10.1126/science.aat5641.

209. Wu, H.; Shen, X.; Huang, L.; Zeng, Y.; Gao, Y.; Shao, L.; Lu, B.; Zhong, Y.; Miao, B.; Xu, Y.; et al. Genotyping single-sperm cells by universal MARSALA enables the acquisition of linkage information for combined pre-implantation genetic diagnosis and genome screening. J. Assist. Reprod. Genet. 2018, 35 (6), 1071-1078. DOI: 10.1007/s10815-018-1158-9.

208. Tan, L.; Xie, X. S. A near-complete spatial map of olfactory receptors in the mouse main olfactory epithelium. Chem. Senses 2018, 43 (6), 427-432. DOI: 10.1093/chemse/bjy030.

207. Ge, H.; Wu, P.; Qian, H.; Xie, X. S. Relatively slow stochastic gene-state switching in the presence of positive feedback significantly broadens the region of bimodality through stabilizing the uninduced phenotypic state. PLoS Comput. Biol. 2018, 14 (3), e1006051. DOI: 10.1371/journal.pcbi.1006051.

206. Chen, Z.; Zhou, W.; Qiao, S.; Kang, L.; Duan, H.; Xie, X. S.; Huang, Y. Highly accurate fluorogenic DNA sequencing with information theory–based error correction. Nat. Biotechnol. 2017, 35 (12), 1170-1178. DOI: 10.1038/nbt.3982.

205. Xu, J.; Zhang, Z.; Niu, W.; Yang, Q.; Yao, G.; Shi, S.; Jin, H.; Song, W.; Chen, L.; Zhang, X.; et al. Mapping allele with resolved carrier status of Robertsonian and reciprocal translocation in human preimplantation embryos. Proc. Natl. Acad. Sci. U.S.A. 2017, 114 (41), E8695-E8702. DOI: 10.1073/pnas.1715053114.

204. Gao, Y.; Ni, X.; Guo, H.; Su, Z.; Ba, Y.; Tong, Z.; Guo, Z.; Yao, X.; Chen, X.; Yin, J.; et al. Single-cell sequencing deciphers a convergent evolution of copy number alterations from primary to circulating tumor cells. Genome Res. 2017, 27 (8), 1312-1322. DOI: 10.1101/gr.216788.116.

203. Chen, C.; Xing, D.; Tan, L.; Li, H.; Zhou, G.; Huang, L.; Xie, X. S. Single-cell whole-genome analyses by Linear Amplification via Transposon Insertion (LIANTI). Science 2017, 356 (6334), 189-194. DOI: 10.1126/science.aak9787.

202. Zhou, Y.; Wang, P.; Tian, F.; Gao, G.; Huang, L.; Wei, W.; Xie, X. S. Painting a specific chromosome with CRISPR/Cas9 for live-cell imaging. Cell Res. 2017, 27 (2), 298-301. DOI: 10.1038/cr.2017.9.

201. Yang, W.; Li, A.; Suo, Y.; Lu, F. K.; Sunney Xie, X. Simultaneous two-color stimulated Raman scattering microscopy by adding a fiber amplifier to a 2 ps OPO-based SRS microscope. Opt. Lett. 2017, 42 (3), 523-526. DOI: 10.1364/OL.42.000523.

200. Fu, D.; Yang, W.; Xie, X. S. Label-free imaging of neurotransmitter acetylcholine at neuromuscular junctions with stimulated Raman scattering. J. Am. Chem. Soc. 2017, 139 (2), 583-586. DOI: 10.1021/jacs.6b10727.

199. Tian, F.; Yang, W.; Mordes, D. A.; Wang, J.-Y.; Salameh, J. S.; Mok, J.; Chew, J.; Sharma, A.; Leno Duran, E.; Suzuki-Uematsu, S.; et al. Monitoring peripheral nerve degeneration in ALS by label-free stimulated Raman scattering imaging. Nat. Commun. 2016, 7 (1), 13283. DOI: 10.1038/ncomms13283.

198. Xu, J.; Fang, R.; Chen, L.; Chen, D.; Xiao, J.-P.; Yang, W.; Wang, H.; Song, X.; Ma, T.; Bo, S.; et al. Noninvasive chromosome screening of human embryos by genome sequencing of embryo culture medium for in vitro fertilization. Proc. Natl. Acad. Sci. U.S.A. 2016, 113 (42), 11907-11912. DOI: 10.1073/pnas.1613294113.

197. Lee, D. F.; Lu, J.; Chang, S.; Loparo, J. J.; Xie, X. S. Mapping DNA polymerase errors by single-molecule sequencing. Nucleic Acids Res. 2016, 44 (13), e118-e118. DOI: 10.1093/nar/gkw436.

196. Lu, F.; Calligaris, D.; Olubiyi, O. I.; Norton, I.; Yang, W.; Santagata, S.; Xie, X. S.; Golby, A. J.; Agar, N. Y. R. Label-free neurosurgical pathology with stimulated Raman imaging. Cancer Res. 2016, 76 (12), 3451-3462. DOI: 10.1158/0008-5472.CAN-16-0270.

195. Pu, Y.; Zhao, Z.; Li, Y.; Zou, J.; Ma, Q.; Zhao, Y.; Ke, Y.; Zhu, Y.; Chen, H.; Baker, Matthew A. B.; et al. Enhanced efflux activity facilitates drug tolerance in dormant bacterial cells. Mol. Cell 2016, 62 (2), 284-294. DOI: 10.1016/j.molcel.2016.03.035.

194. Zhao, Z. W.; Xie, X. S.; Ge, H. Nonequilibrium relaxation of conformational dynamics facilitates catalytic reaction in an elastic network model of T7 DNA polymerase. J. Phys. Chem. B 2016, 120 (11), 2869-2877. DOI: 10.1021/acs.jpcb.5b11002.

193. Yan, L.; Huang, L.; Xu, L.; Huang, J.; Ma, F.; Zhu, X.; Tang, Y.; Liu, M.; Lian, Y.; Liu, P.; et al. Live births after simultaneous avoidance of monogenic diseases and chromosome abnormality by next-generation sequencing with linkage analyses. Proc. Natl. Acad. Sci. U.S.A. 2015, 112 (52), 15964-15969. DOI: 10.1073/pnas.1523297113.

192. Tan, L.; Li, Q.; Xie, X. S. Olfactory sensory neurons transiently express multiple olfactory receptors during development. Mol. Syst. Biol. 2015, 11 (12), 844. DOI: 10.15252/msb.20156639.

191. Cheng, J. X.; Xie, X. S. Vibrational spectroscopic imaging of living systems: an emerging platform for biology and medicine. Science 2015, 350 (6264), aaa8870. DOI: 10.1126/science.aaa8870.

190. Gu, C.; Zhang, J.; Yang, Y. I.; Chen, X.; Ge, H.; Sun, Y.; Su, X.; Yang, L.; Xie, S.; Gao, Y. Q. DNA structural correlation in short and long ranges. J. Phys. Chem. B 2015, 119 (44), 13980-13990. DOI: 10.1021/acs.jpcb.5b06217.

189. Ji, M.; Lewis, S.; Camelo-Piragua, S.; Ramkissoon, S. H.; Snuderl, M.; Venneti, S.; Fisher-Hubbard, A.; Garrard, M.; Fu, D.; Wang, A. C.; et al. Detection of human brain tumor infiltration with quantitative stimulated Raman scattering microscopy. Sci Transl Med 2015, 7 (309), 309ra163-309ra163. DOI: 10.1126/scitranslmed.aab0195.

188. Fu, Y.; Li, C.; Lu, S.; Zhou, W.; Tang, F.; Xie, X. S.; Huang, Y. Uniform and accurate single-cell sequencing based on emulsion whole-genome amplification. Proc. Natl. Acad. Sci. U.S.A. 2015, 112 (38), 11923-11928. DOI: 10.1073/pnas.1513988112.

187. Lu, F. K.; Basu, S.; Igras, V.; Hoang, M. P.; Ji, M.; Fu, D.; Holtom, G. R.; Neel, V. A.; Freudiger, C. W.; Fisher, D. E.; et al. Label-free DNA imaging in vivo with stimulated Raman scattering microscopy. Proc. Natl. Acad. Sci. U.S.A. 2015, 112 (37), 11624-11629. DOI: 10.1073/pnas.1515121112.

186. Huang, L.; Ma, F.; Chapman, A.; Lu, S.; Xie, X. S. Single-cell whole-genome amplification and sequencing: methodology and applications. Annu Rev Genomics Hum Genet 2015, 16 (1), 79-102. DOI: 10.1146/annurev-genom-090413-025352.

185. Xie, X. S. Single molecules meet genomics: pinpointing precision medicine. JAMA 2015, 313 (20), 2021-2022. DOI: 10.1001/jama.2015.5533.

184. Chen, Z.; Duan, H.; Qiao, S.; Zhou, W.; Qiu, H.; Kang, L.; Xie, X. S.; Huang, Y. Fluorogenic sequencing using halogen-fluorescein-labeled nucleotides. Chembiochem 2015, 16 (8), 1153-1157. DOI: 10.1002/cbic.201500117.

183. Chapman, A. R.; He, Z.; Lu, S.; Yong, J.; Tan, L.; Tang, F.; Xie, X. S. Single cell transcriptome amplification with MALBAC. PLoS One 2015, 10 (3), e0120889. DOI: 10.1371/journal.pone.0120889.

182. Ge, H.; Qian, H.; Xie, X. S. Stochastic phenotype transition of a single cell in an Intermediate region of gene state switching. Phys. Rev. Lett. 2015, 114 (7), 078101. DOI: 10.1103/PhysRevLett.114.078101.

181. Chen, H.; Shiroguchi, K.; Ge, H.; Xie, X. S. Genome-wide study of mRNA degradation and transcript elongation in Escherichia coli. Mol. Syst. Biol. 2015, 11 (1), 781. DOI: 10.15252/msb.20145794.

180. Kou, S. C.; Xie, X. S. Generalized langevin equation with fractional gaussian noise: subdiffusion within a single protein molecule. Phys. Rev. Lett. 2004, 93 (18), 180603. DOI: 10.1103/PhysRevLett.93.180603.

179. Jin, J.; Lian, T.; Sunney Xie, X.; Su, X.-D. High-accuracy mapping of protein binding stability on nucleosomal DNA using a single-molecule method. J Mol Cell Biol 2014, 6 (5), 438-440. DOI: 10.1093/jmcb/mju033.

178. Chong, S.; Chen, C.; Ge, H.; Xie, X. S. Mechanism of transcriptional bursting in bacteria. Cell 2014, 158 (2), 314-326. DOI: 10.1016/j.cell.2014.05.038.

177. Fu, D.; Zhou, J.; Zhu, W. S.; Manley, P. W.; Wang, Y. K.; Hood, T.; Wylie, A.; Xie, X. S. Imaging the intracellular distribution of tyrosine kinase inhibitors in living cells with quantitative hyperspectral stimulated Raman scattering. Nat. Chem. 2014, 6 (7), 614-622. DOI: 10.1038/nchem.1961.

176. Fu, D.; Yu, Y.; Folick, A.; Currie, E.; Farese, R. V., Jr.; Tsai, T. H.; Xie, X. S.; Wang, M. C. In vivo metabolic fingerprinting of neutral lipids with hyperspectral stimulated Raman scattering microscopy. J. Am. Chem. Soc. 2014, 136 (24), 8820-8828. DOI: 10.1021/ja504199s.

175. Wang, S.; Moffitt, J. R.; Dempsey, G. T.; Xie, X. S.; Zhuang, X. Characterization and development of photoactivatable fluorescent proteins for single-molecule–based superresolution imaging. Proc. Natl. Acad. Sci. U.S.A. 2014, 111 (23), 8452-8457. DOI: 10.1073/pnas.1406593111.

174. Fu, D.; Xie, X. S. Reliable cell segmentation based on spectral phasor analysis of hyperspectral stimulated Raman scattering imaging data. Anal. Chem. 2014, 86 (9), 4115-4119. DOI: 10.1021/ac500014b.

173. Bentley, J. N.; Ji, M.; Xie, X. S.; Orringer, D. A. Real-time image guidance for brain tumor surgery through stimulated Raman scattering microscopy. Expert Rev Anticancer Ther 2014, 14 (4), 359-361. DOI: 10.1586/14737140.2013.877844.

172. Freudiger, C. W.; Yang, W.; Holtom, G. R.; Peyghambarian, N.; Xie, X. S.; Kieu, K. Q. Stimulated Raman scattering microscopy with a robust fibre laser source. Nat. Photonics 2014, 8 (2), 153-159. DOI: 10.1038/nphoton.2013.360.

171. Zhao, Z. W.; Roy, R.; Gebhardt, J. C. M.; Suter, D. M.; Chapman, A. R.; Xie, X. S. Spatial organization of RNA polymerase II inside a mammalian cell nucleus revealed by reflected light-sheet superresolution microscopy. Proc. Natl. Acad. Sci. U.S.A. 2014, 111 (2), 681-686. DOI: 10.1073/pnas.1318496111.

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74. Evans, C. L.; Potma, E. O.; Xie, X. S. Coherent anti-Stoke Raman scattering spectral interferometry: determination of the real and imaginary components of nonlinear susceptibility χ(3) for vibrational microscopy. Opt. Lett. 2004, 29 (24), 2923-2925. DOI: 10.1364/OL.29.002923.

73. Cotlet, M.; Masuo, S.; Luo, G.; Hofkens, J.; Van der Auweraer, M.; Verhoeven, J.; Müllen, K.; Xie, X. S.; De Schryver, F. Probing conformational dynamics in single donor-acceptor synthetic molecules by means of photoinduced reversible electron transfer. Proc. Natl. Acad. Sci. U.S.A. 2004, 101 (40), 14343-14348. DOI: 10.1073/pnas.0406119101.

72. Nan, X.; Yang, W. Y.; Xie, X. S. CARS microscopy lights up lipids in living cells. Biophotonics Int. 2004, 11 (8), 44-47.

71. Rech, I.; Guobin, L.; Ghioni, M.; Haw, Y.; Xiaoliang Sunney, X.; Cova, S. Photon-timing detector module for single-molecule spectroscopy with 60-ps resolution. IEEE J Sel Top Quantum Electron 2004, 10 (4), 788-795. DOI: 10.1109/JSTQE.2004.833975.

70. Potma, E. O.; Xie, X. S.; Muntean, L.; Preusser, J.; Jones, D.; Ye, J.; Leone, S. R.; Hinsberg, W. D.; Schade, W. Chemical imaging of photoresists with coherent anti-Stoke Raman scattering (CARS) microscopy. J. Phys. Chem. B 2004, 108 (4), 1296-1301. DOI: 10.1021/jp030903p.

69. Cheng, J. X.; Xie, X. S. Coherent anti-Stoke Raman scattering microscopy:  instrumentation, theory, and applications. J. Phys. Chem. B 2004, 108 (3), 827-840. DOI: 10.1021/jp035693v.

68. Dufresne, E. R.; Corwin, E. I.; Greenblatt, N. A.; Ashmore, J.; Wang, D. Y.; Dinsmore, A. D.; Cheng, J. X.; Xie, X. S.; Hutchinson, J. W.; Weitz, D. A. Flow and fracture in drying nanoparticle suspensions. Phys. Rev. Lett. 2003, 91 (22), 224501. DOI: 10.1103/PhysRevLett.91.224501.

67. Pautot, S.; Frisken, B. J.; Cheng, J. X.; Xie, X. S.; Weitz, D. A. Spontaneous formation of lipid structures at oil/water/lipid interfaces. Langmuir 2003, 19 (24), 10281-10287. DOI: 10.1021/la034532f.

66. Nan, X.; Cheng, J. X.; Xie, X. S. Vibrational imaging of lipid droplets in live fibroblast cells with coherent anti-Stoke Raman scattering microscopy. J. Lipid Res. 2003, 44 (11), 2202-2208. DOI: 10.1194/jlr.D300022-JLR200.

65. Yang, H.; Luo, G.; Karnchanaphanurach, P.; Louie, T. M.; Rech, I.; Cova, S.; Xun, L.; Xie, X. S. Protein conformational dynamics probed by single-molecule electron transfer. Science 2003, 302 (5643), 262-266. DOI: 10.1126/science.1086911.

64. Potma, E. O.; Evans, C.; Xie, X. S.; Jones, R. J.; Ye, J. Picosecond-pulse amplification with an external passive optical cavity. Opt. Lett. 2003, 28 (19), 1835-1837. DOI: 10.1364/OL.28.001835.

63. Potma, E. O.; Xie, X. S. Detection of single lipid bilayers with coherent anti-Stoke Raman scattering (CARS) microscopy. J Raman Spectrosc 2003, 34 (9), 642-650. DOI: 10.1002/jrs.1045.

62. van Oijen, A. M.; Blainey, P. C.; Crampton, D. J.; Richardson, C. C.; Ellenberger, T.; Xie, X. S. Single-molecule kinetics of λ exonuclease reveal base dependence and dynamic disorder. Science 2003, 301 (5637), 1235-1238. DOI: 10.1126/science.1084387.

61. Cheng, J. X.; Pautot, S.; Weitz, D. A.; Xie, X. S. Ordering of water molecules between phospholipid bilayers visualized by coherent anti-Stokes Raman scattering microscopy. Proc. Natl. Acad. Sci. U.S.A. 2003, 100 (17), 9826-9830. DOI: 10.1073/pnas.1732202100.

60. Louie, T. M.; Xie, X. S.; Xun, L. Coordinated production and utilization of FADH2 by NAD(P)H−flavin oxidoreductase and 4-hydroxyphenylacetate 3-monooxygenase. Biochemistry 2003, 42 (24), 7509-7517. DOI: 10.1021/bi034092r.

59. Hartschuh, A.; Sánchez, E. J.; Xie, X. S.; Novotny, L. High-resolution near-field Raman microscopy of single-walled carbon nanotubes. Phys. Rev. Lett. 2003, 90 (9), 095503. DOI: 10.1103/PhysRevLett.90.095503.

58. Yang, H.; Xie, X. S. Probing single-molecule dynamics photon by photon. J. Chem. Phys. 2002, 117 (24), 10965-10979. DOI: 10.1063/1.1521154.

57. Xie, X. S. Single-molecule approach to dispersed kinetics and dynamic disorder: probing conformational fluctuation and enzymatic dynamics. J. Chem. Phys. 2002, 117 (24), 11024-11032. DOI: 10.1063/1.1521159.

56. Yang, H.; Xie, X. S. Statistical approaches for probing single-molecule dynamics photon-by-photon. Chem. Phys. 2002, 284 (1), 423-437. DOI: 10.1016/S0301-0104(02)00672-9.

55. Sánchez, E. J.; Krug, J. T.; Xie, X. S. Ion and electron beam assisted growth of nanometric SimOn structures for near-field microscopy. Rev. Sci. Instrum. 2002, 73 (11), 3901-3907. DOI: 10.1063/1.1511801.

54. Louie, T. M.; Yang, H.; Karnchanaphanurach, P.; Xie, X. S.; Xun, L. FAD is a preferred substrate and an Inhibitor of escherichia coli general NAD(P)H: flavin oxidoreductase. J. Biol. Chem. 2002, 277 (42), 39450-39455. DOI: 10.1074/jbc.M206339200.

53. Cheng, J. X.; Potma, E. O.; Xie, S. X. Coherent anti-Stoke Raman scattering correlation spectroscopy:  probing dynamical processes with chemical selectivity. J. Phys. Chem. A 2002, 106 (37), 8561-8568. DOI: 10.1021/jp025774b.

52. Jones, D. J.; Potma, E. O.; Cheng, J.; Burfeindt, B.; Pang, Y.; Ye, J.; Xie, X. S. Synchronization of two passively mode-locked, picosecond lasers within 20 fs for coherent anti-Stoke raman scattering microscopy. Rev. Sci. Instrum. 2002, 73 (8), 2843-2848. DOI: 10.1063/1.1492001.

51. Cheng, J. X.; Volkmer, A.; Book, L. D.; Xie, X. S. Multiplex coherent anti-Stoke Raman scattering microspectroscopy and study of lipid vesicles. J. Phys. Chem. B 2002, 106 (34), 8493-8498. DOI: 10.1021/jp025771z.

50. Potma, E. O.; Jones, D. J.; Cheng, J. X.; Xie, X. S.; Ye, J. High-sensitivity coherent anti-Stoke raman scattering microscopy with two tightly synchronized picosecond lasers. Opt. Lett. 2002, 27 (13), 1168-1170. DOI: 10.1364/OL.27.001168.

49. Cheng, J. X.; Xie, X. S. Green’s function formulation for third-harmonic generation microscopy. J. Opt. Soc. Am. B 2002, 19 (7), 1604-1610. DOI: 10.1364/JOSAB.19.001604.

48. Cheng, J. X.; Jia, Y. K.; Zheng, G.; Xie, X. S. Laser-scanning coherent anti-Stoke Raman scattering microscopy and applications to cell biology. Biophys. J. 2002, 83 (1), 502-509. DOI: 10.1016/S0006-3495(02)75186-2.

47. Krug, J. T.; Sánchez, E. J.; Xie, X. S. Design of near-field optical probes with optimal field enhancement by finite difference time domain electromagnetic simulation. J. Chem. Phys. 2002, 116 (24), 10895-10901. DOI: 10.1063/1.1479723.

46. Cheng, J. X.; Volkmer, A.; Xie, X. S. Theoretical and experimental characterization of coherent anti-Stoke Raman scattering microscopy. J. Opt. Soc. Am. B 2002, 19 (6), 1363-1375. DOI: 10.1364/JOSAB.19.001363.

45. Volkmer, A.; Book, L. D.; Xie, X. S. Time-resolved coherent anti-Stoke Raman scattering microscopy: imaging based on raman free induction decay. Appl. Phys. Lett. 2002, 80 (9), 1505-1507. DOI: 10.1063/1.1456262.

44. Xie, S. Single-molecule approach to enzymology. Single mol. 2001, 2 (4), 229-236. DOI: 10.1002/1438-5171(200112)2:4<229::AID-SIMO229>3.0.CO;2-9.

43. Cheng, J. X.; Book, L. D.; Xie, X. S. Polarization coherent anti-Stoke Raman scattering microscopy. Opt. Lett. 2001, 26 (17), 1341-1343. DOI: 10.1364/OL.26.001341.

42. Volkmer, A.; Cheng, J. X.; Sunney Xie, X. Vibrational imaging with high sensitivity via epidetected coherent anti-Stoke Raman scattering microscopy. Phys. Rev. Lett. 2001, 87 (2), 023901. DOI: 10.1103/PhysRevLett.87.023901.

41. Cheng, J. X.; Volkmer, A.; Book, L. D.; Xie, X. S. An epi-detected coherent anti-Stoke Raman scattering (E-CARS) microscope with high spectral resolution and high sensitivity. J. Phys. Chem. B 2001, 105 (7), 1277-1280. DOI: 10.1021/jp003774a.

40. 应立明, 谢. 单分子的光学检测及应用. 大学化学 1999, 14 (5), 1-6. DOI: 10.3866/pku.Dxhx19990501.

39. Schenter, G. K.; Lu, H. P.; Xie, X. S. Statistical analyses and theoretical models of single-molecule enzymatic dynamics. J. Phys. Chem. A 1999, 103, 10477-10488.

38. Bai, C.; Wang, C.; Xie, X. S.; Wolynes, P. G. Single molecule physics and chemistry. Proc. Natl. Acad. Sci. U.S.A. 1999, 96 (20), 11075-11076. DOI: 10.1073/pnas.96.20.11075.

37. Lu, H. P.; Xie, X. S. Single-molecule spectroscopy studies of molecular dynamics in chemical and biological systems. Z Phys Chem (N F) 1999, 212 (1), 59-66. DOI: 10.1524/zpch.1999.212.Part_1.059.

36. Xie, X. S.; Lu, H. P. Single-molecule enzymology. J. Biol. Chem. 1999, 274 (23), 15967-15970. DOI: 10.1074/jbc.274.23.15967.

35. Zumbusch, A.; Holtom, G. R.; Xie, X. S. Three-dimensional vibrational imaging by coherent anti-Stoke Raman scattering. Phys. Rev. Lett. 1999, 82 (20), 4142-4145. DOI: 10.1103/PhysRevLett.82.4142.

34. Sánchez, E. J.; Novotny, L.; Xie, X. S. Near-field fluorescence microscopy based on two-photon excitation with metal tips. Phys. Rev. Lett. 1999, 82 (20), 4014-4017. DOI: 10.1103/PhysRevLett.82.4014.

33. Lu, H. P.; Xun, L.; Xie, X. S. Single-molecule enzymatic dynamics. Science 1998, 282 (5395), 1877-1882. DOI: 10.1126/science.282.5395.1877.

32. Ying, L.; Xie, X. S. Fluorescence spectroscopy, exciton dynamics, and photochemistry of single allophycocyanin trimers. J. Phys. Chem. B 1998, 102 (50), 10399-10409. DOI: 10.1021/jp983227d.

31. Xie, X. S.; Trautman, J. K. Optical studies of single molecules at room temperature. Annu Rev Phys Chem 1998, 49 (1), 441-480. DOI: 10.1146/annurev.physchem.49.1.441.

30. Sánchez, E. J.; Novotny, L.; Holtom, G. R.; Xie, X. S. Room-temperature fluorescence imaging and spectroscopy of single molecules by two-photon excitation. J. Phys. Chem. A 1998, 102 (25), 4948-4948. DOI: 10.1021/jp981638o.

29. Novotny, L.; Sánchez, E. J.; Xie, X. S. Near-field optical imaging using metal tips illuminated by higher-order Hermite–Gaussian beams. Ultramicroscopy 1998, 71 (1), 21-29. DOI: 10.1016/S0304-3991(97)00077-6.

28. Novotny, L.; Bian, R. X.; Xie, X. S. Theory of nanometric optical tweezers. Phys. Rev. Lett. 1997, 79 (4), 645-648. DOI: 10.1103/PhysRevLett.79.645.

27. Lu, H. P.; Xie, X. S. Single-molecule kinetics of interfacial electron transfer. J. Phys. Chem. B 1997, 101 (15), 2753-2757. DOI: 10.1021/jp9634518.

26. Lu, H. P.; Xie, X. S. Single-molecule spectral fluctuations at room temperature. Nature 1997, 385, 143-146.

25. Xie, X. S. Single-molecule spectroscopy and dynamics at room temperature. Acc. Chem. Res. 1996, 29 (12), 598-606. DOI: 10.1021/ar950246m.

24. Bian, R. X.; Dunn, R. C.; Xie, X. S.; Leung, P. T. Single molecule emission characteristics in near-field microscopy. Phys. Rev. Lett. 1995, 75 (26), 4772-4775. DOI: 10.1103/PhysRevLett.75.4772.

23. Holtom, G. R.; Crowell, R. A.; Xie, X. S. High-repetition-rate femtosecond optical parametric oscillator–amplifier system near 3 μm. J. Opt. Soc. Am. B 1995, 12 (9), 1723-1731. DOI: 10.1364/JOSAB.12.001723.

22. Dunn, R. C.; Xie, X. S. Characterization of the temporal behavior of ultrashort pulses emerging from a near-field fiber probe. Ultramicroscopy 1995, 57 (2), 169-172. DOI: 10.1016/0304-3991(94)00130-F.

21. Dunn, R. C.; Allen, E. V.; Joyce, S. A.; Anderson, G. A.; Sunney Xie, X. Near-field fluorescent imaging of single proteins. Ultramicroscopy 1995, 57 (2), 113-117. DOI: 10.1016/0304-3991(94)00121-3.

20. Crowell, R. A.; Holtom, G. R.; Xie, X. S. Infrared free induction decay of liquid water molecules. J. Phys. Chem. 1995, 99 (7), 1840-1842. DOI: 10.1021/j100007a009.

19. Xie, X. S.; Dunn, R. C. Probing single molecule dynamics. Science 1994, 265 (5170), 361-364. DOI: 10.1126/science.265.5170.361.

18. Du, M.; Xie, X.; Mets, L.; Fleming, G. R. Direct observation of ultrafast energy-transfer processes in light harvesting complex II. J. Phys. Chem. 1994, 98 (17), 4736-4741. DOI: 10.1021/j100068a041.

17. Dunn, R. C.; Holtom, G. R.; Mets, L.; Xie, X. S. Near-field fluorescence imaging and fluorescence lifetime measurement of light harvesting complexes in intact photosynthetic membranes. J. Phys. Chem. 1994, 98 (12), 3094-3098. DOI: 10.1021/j100063a010.

16. Du, M.; Xie, X.; Jia, Y.; Mets, L.; Fleming, G. R. Direct observation of ultrafast energy transfer in PSI core antenna. Chem. Phys. Lett. 1993, 201 (5), 535-542. DOI: 10.1016/0009-2614(93)85113-3.

15. Du, M.; Rosenthal, S. J.; Xie, X.; DiMagno, T. J.; Schmidt, M.; Hanson, D. K.; Schiffer, M.; Norris, J. R.; Fleming, G. R. Femtosecond spontaneous-emission studies of reaction centers from photosynthetic bacteria. Proc. Natl. Acad. Sci. U.S.A. 1992, 89 (18), 8517-8521. DOI: doi:10.1073/pnas.89.18.8517.

14. Lewis, J. W.; Goldbeck, R. A.; Kliger, D. S.; Xie, X.; Dunn, R. C.; Simon, J. D. Time-resolved circular dichroism spectroscopy: experiment, theory, and applications to biological systems. J. Phys. Chem. 1992, 96 (13), 5243-5254. DOI: 10.1021/j100192a016.

13. Rosenthal, S. J.; Xie, X.; Du, M.; Fleming, G. R. Femtosecond solvation dynamics in acetonitrile: observation of the inertial contribution to the solvent response. J. Chem. Phys. 1991, 95 (6), 4715-4718. DOI: 10.1063/1.461742.

12. Xie, X.; Simon, J. D. Protein conformational relaxation following photodissociation of carbon monoxide from carbonmonoxymyoglobin: picosecond circular dichroism and absorption studies. Biochemistry 1991, 30 (15), 3682-3692. DOI: 10.1021/bi00229a013.

11. Xie, X.; Simon, J. D. A picosecond circular dichroism study of photosynthetic reaction centers from Rhodobacter sphaeroides. Biochim Biophys Acta Bioenerg 1991, 1057 (1), 131-139. DOI: 10.1016/S0005-2728(05)80093-3.

10. Xie, X.; Simon, J. D. Picosecond magnetic circular dichroism spectroscopy. J. Phys. Chem. 1990, 94 (21), 8014-8016. DOI: 10.1021/j100384a006.

9. Xie, X.; Simon, J. D. Picosecond time-resolved circular dichroism study of protein relaxation in myoglobin following photodissociation of carbon monoxide. J. Am. Chem. Soc. 1990, 112 (21), 7802-7803. DOI: 10.1021/ja00177a054.

8. Xie, X.; Simon, J. D. Picosecond circular dichroism spectroscopy: a Jones matrix analysis. J. Opt. Soc. Am. B 1990, 7 (8), 1673-1684. DOI: 10.1364/JOSAB.7.001673.

7. Xie, X.; Simon, J. D. Picosecond time resolved absorption studies of the solvation of Cr(CO)5 in alcohols: a unimolecular kinetic model for the formation of Cr(CO)5(OHR) from photogenerated Cr(CO)5(ROH). J. Am. Chem. Soc. 1990, 112 (3), 1130-1136. DOI: 10.1021/ja00159a037.

6. Xie, X.; Simon, J. D. Picosecond time‐resolved circular dichroism spectroscopy: experimental details and applications. Rev. Sci. Instrum. 1989, 60 (8), 2614-2627. DOI: 10.1063/1.1140681.

5. Xie, X.; Simon, J. D. Photodissociation of chromium hexacarbonyl and hexakis(phenyl isocyanide)chromium in tetrahydrofuran. J. Phys. Chem. 1989, 93 (11), 4401-4404. DOI: 10.1021/j100348a006.

4. Xie, X.; Simon, J. D. High energy and tunable picosecond laser pulses at 1 kHz: synchronously pumping a dye laser with a mode-locked, Q-switched and cavity dumped Nd:YAG laser system. Opt. Commun. 1989, 69 (3), 303-307. DOI: 10.1016/0030-4018(89)90121-1.

3. Simon, J. D.; Xie, X. Photodissociation of chromium hexacarbonyl in 1-propanol and 2-propanol: effect of solvent structure on the mechanisms of formation of Cr(CO)5(OHR) from photogenerated Cr(CO)5(ROH). J. Phys. Chem. 1989, 93 (1), 291-293. DOI: 10.1021/j100338a059.

2. Simon, J. D.; Xie, X. Time-resolved studies of solvation: the photodissociation of hexacarbonylchromium in pentanol. J. Phys. Chem. 1987, 91 (22), 5538-5540. DOI: 10.1021/j100306a007.

1. Simon, J. D.; Xie, X. Photodissociation of chromium hexacarbonyl in solution: direct observation of the formation of pentacarbonyl (methanol) chromium. J. Phys. Chem. 1986, 90 (26), 6751-6753. DOI: 10.1021/j100284a005.

Full List of US Patents

15. Xie, X. S.; Shiroguchi, K.; Sims, P. A.; Jia, T. Z. Single cell nucleic acid detection and analysis. US-11608527-B2, 2023.

14. Xie, X. S.; Xing, D.; Chang, C.-H.; Tan, L. Multiplex end-tagging amplification of nucleic acids. US-11530436-B2, 2022.

13. Chen, C.; Xing, D.; Xie, X. S. Methods of amplifying nucleic acid sequences mediated by transposase/transposon dna complexes. US-10894980-B2, 2021.

12. Mangel, W. F.; Blainey, P.; Graziano, V.; Herrmann, A.; McGrath, W. J.; Oijen, A. M. V.; Xie, X. S. Molecular sleds and uses thereof. US-10065989-B2, 2018.

11. Kieu, K.; Peyghambarian, N.; Xie, X. S.; Freudiger, C. W.; Fu, D. Laser illumination systems and methods for dual-excitation wavelength non-linear optical microscopy and micro-spectroscopy systems. US-9634454-B1, 2017.

10. Xie, X. S.; Zong, C.; Lu, S. Methods of amplifying whole genome of a single cell. US-9617598-B2, 2017.

9. Xie, X. S.; Freudiger, C.; Min, W. Systems and methods for selective detection and imaging in coherent Raman microscopy by spectral excitation shaping. US-9285575-B2, 2016.

8. Xie, S. X.; Freudiger, C. W.; Saar, B. G. Systems and methods providing efficient detection of back-scattered illumination in modulation transfer microscopy or micro-spectroscopy. US-8681331-B2, 2014.

7. Xie, X. S.; Freudiger, C.; Min, W. Microscopy imaging system and method employing stimulated Raman spectroscopy as a contrast mechanism. US-8027032-B2, 2011.

6. Gankkhanov, F.; Carrasco, S.; Xie, X. S. System and method for providing a tunable optical parametric oscillator laser system that provides dual frequency output for non-linear vibrational spectroscopy and microscopy. US-7616304-B2, 2009.

5. Murugkar, S.; Anis, H.; Xie, X. S.; Evans, C. Pathogen detection using coherent anti-Stokes Raman scattering microscopy. US-7542137-B2, 2009.

4. Xie, X. S.; Légaré, F.; Ganikhanov, F.; Evans, C. System and method for coherent anti-Stokes raman scattering endoscopy. US-7414729-B2, 2008.

3. Potma, E. O.; Evans, C.; Xie, X. S. Phase sensitive heterodyne coherent anti-Stokes Raman scattering micro-spectroscopy and microscopy. US-7388668-B2, 2008.

2. Xie, X. S.; Ganikhanov, F.; Evans, C. System and method for high sensitivity vibrational imaging with frequency modulation coherent anti-Stokes Raman scattering analyses. US-7352458-B2, 2008.

1. Holtom, G. R.; Xie, X. S.; Zumbusch, A. Nonlinear vibrational microscopy. US-6108081-A, 2000.

Books

1. Cheng, J. X.; Xie, X. S. Coherent Raman scattering microscopy; CRC Press, 2012.

Book Chapters

10. Blanshard, R. C.; Chen, C.; Xie, X. S.; Hoffmann, E. R. Single cell genomics to study DNA and chromosome changes in human gametes and embryos. In Methods in Cell Biology, Maiato, H., Schuh, M. Eds.; Vol. 144; Academic Press, 2018; pp 441-457.

9. Xie, X. S. Enzymology and life at the single molecule level. In Single Molecule Spectroscopy in Chemistry, Physics and Biology: Nobel Symposium, Gräslund, A., Rigler, R., Widengren, J. Eds.; Springer Berlin Heidelberg, 2010; pp 435-448.

8. Xiao, J.; Elf, J.; Li, G. W.; Yu, J.; Xie, X. S. Imaging gene expression in living cells at the single molecule level. In Single Molecules: A Laboratory Manual, Selvin, P. R., Ha, T. Eds.; Cold Spring Harbor Laboratory Press, 2008; pp 149-170.

7. Schroeder, C. M.; Blainey, P. C.; Kim, S.; Xie, X. S. Hydrodynamic flow-stretching assay for single-molecule studies of nucleic acid–protein interactions. In Single Molecules: A Laboratory Manual, Selvin, P. R., Ha, T. Eds.; Cold Spring Harbor Laboratory Press, 2008; pp 461-492.

6. Potma, E.; Xie, S. X. Theory of spontaneous and coherent raman scattering. In Handbook of Biological Nonlinear Optical Microscopy, Masters, B. R., So, P. Eds.; Oxford University Press, 2008; pp 164-186.

5. Xie, X. S.; Cheng, J.; Potma, E. Coherent anti-Stokes Raman scattering microscopy. In Handbook of Biological Confocal Microscopy, Pawley, J. B. Ed.; Springer US, 2006; pp 595-606.

4. Xie, X. S.; Bian, R. X.; Dunn, R. C. Near-field microscopy and spectroscopy of single molecules, single proteins and biological membranes. In Focus on Multidimensional Microscopy, World Scientific Publishing Co., 1999; pp 263-293.

3. Dunn, R. C.; Xie, X.; Simon, J. D. Real-time spectroscopic techniques for probing conformational dynamics of heme proteins. In Methods in Enzymology, Vol. 226; Academic Press, 1993; pp 177-198.

2. Rosenthal, S. J.; Scherer, N. F.; Cho, M.; Xie, X.; Schmidt, M. E.; Fleming, G. R. Femtosecond solvent dynamics studied by time resolved fluorescence and transient birefringence. In Eighth International Conference on Ultrafast Phenomena, Antibes - Juan-Les-Pins, 1992; Optica Publishing Group: p FC13.

1. Xie, X.; Simon, J. D. Picosecond polarization studies of protein relaxation. In ultrafast phenomena VII, Berlin, Heidelberg, 1990; Harris, C. B., Ippen, E. P., Mourou, G. A., Zewail, A. H., Eds.; Springer Berlin Heidelberg: pp 544-547.